Novel biochemical and functional insights into nuclear Ca21 transport through IP3Rs and RyRs in osteoblasts
نویسندگان
چکیده
OLUGBENGA A. ADEBANJO,1 GOPA BISWAS,2 BALJIT S. MOONGA,1 HINDUPUR K. ANANDATHEERTHAVARADA,2 LI SUN,1 PETER J. R. BEVIS,1 BALI R. SODAM,1 F. ANTHONY LAI,3 NARAYAN G. AVADHANI,2 AND MONE ZAIDI1 1Division of Endocrinology and Metabolism, Mount Sinai School of Medicine, and Bronx Veterans Affairs Geriatric Research Education and Clinical Center, New York 10029; 2School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and 3National Institute of Medical Research, London, NW7 1AA United Kingdom
منابع مشابه
Novel biochemical and functional insights into nuclear Ca(2+) transport through IP(3)Rs and RyRs in osteoblasts.
We report the first biochemical and functional characterization of inositol trisphosphate receptors (IP(3)Rs) and ryanodine receptors (RyRs) in the nuclear membrane of bone-forming (MC3T3-E1) osteoblasts. Intact nuclei fluoresced intensely with anti-RyR (Ab(34)) and anti-IP(3)R (Ab(40)) antisera in a typically peripheral nuclear membrane pattern. Isolated nuclear membranes were next subjected t...
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